Serial dilution lab purpose

A serial dilution is the stepwise dilution of a substance in solution. Consider the example of making the first standard at the concentration of the known, the next calibrant would be 19th the concentration of the known. Tube agglutination method is the recommended method of performing the widal test. The dilution factor chosen for the series of calibration standards is achievable by using serial dilution. The diluted sample is then used as the base solution to make an additional dilution. To perform a serial dilution, a small amount of a wellmixed solution is transferred into a new container, and additional water or other solvent is added to dilute the original solution. Protocol for using serial dilutions in the microbiology lab. Often the standard which is given to you in the lab is far to strong for the experiment and it needs to be diluted. Importance of serial dilution in serological tests. The progression of calibration standard concentration is always a geometric series. The easiest method is to make a series of 1 in 10 dilutions. Usually the dilution factor at each step is constant, resulting in a geometric progression of the. An easy learning guide dilution is the process of making a solution weaker or less concentrated.

A serial dilution is a series of sequential dilutions used to reduce a dense culture of cells to a more usable concentration. In microbiology, serial dilutions log dilutions are used to decrease a bacterial concentration to a required concentration for a specific test method, or to a concentration which is easier to count when plated to an agar plate i have created this guide to provide a better understanding of dilutions and should be used. Upon completion of this lab, the student should be able to proficiently calculate molarities for solutions. A dilution can be performed not only to lower the concentration of the analyte that is being tested, so that it is in range, but also to help eliminate interferences from other substances that may be present in the sample that can. This video shows creation of a series of solutions of different ph by serial dilution. Pdf lab report of microbiology akbar haqi academia. A serial dilution is a series of stepwise dilutions, where the dilution factor is held constant at each step. Serial dilution generally refers to selection preformed in the standard growth regimes typically used in the lab. This exercise is intended to familiarize us with using scientific notation to express big and small number easily and the process of using serial dilutions to make. The levels are measured by using serial dilutions of sera. Dilutions can be important when dealing with an unknown substance. Many laboratory exercises and experiments require one to make serial dilutions of bacterial cultures or virus. Repeat the steps until the cells can be observed under the microscope when the diluted sample was observed. For the solutions to be usable in the lab for a titration, for instance, they must be accurately diluted to a known, lesser concentration.

Your microbiology lab instructor taught you the basics of serial dilution and how to use it to determine the number of cells in an original bacterial culture. First, take a portion of the sample and does serial dilution on it. Serial dilution simulation lab the american biology teacher. As such, microbiologists have employed serial dilution and various plating. Mass per unit volume is often used to report the concentration of proteins and. Serial dilution is a simple yet efficient technique to determine the number of cells or organisms in a concentrated sample. Importance of serial dilution in serological testsserial dilution lab purpose rating. Many laboratory exercises and experiments require one to make serial dilutions of bacterial cultures or virus cultures in order to calculate the concentration of. Serial dilution helps to choose a dilution which is relevant to our experiment.